Micrometer Introduction:
1. Table micro ruler: The table micro ruler is 1 mm long, divided into 100 small grids, each of which is 10 microns. There is a small black ring on the periphery of the scale to find the position of the scale under the microscope, and there is a ring on the scale. Round coverslips for protection. The cover slip is glued to the slide with gum, so avoid contact with xylene. Bench micrometer is the standard for measuring microscopic length. It is not used for direct measurement, but used to calibrate the eye micrometer, so its quality greatly affects the measured microbody.
2. Micrometer ruler: divided into linear micrometer ruler and mesh micrometer ruler. The linear eye micrometer is a circular glass piece with a scale slightly smaller than the inner diameter of the eyepiece tube. The scale is 10 mm long and divided into 100 grids (10: 100). There are several square grid scales on the mesh micrometer, and the mesh micrometer is often used to find the area.
3. Change the objective lens to the required multiple, and adjust the focal length so that the ruled line to the micrometer is clearest. At this time, the micrometer and the micrometer can be seen at the same time in the field of view. Move the stage to move the micrometer ruler below the micrometer ruler to prevent the scale on the latter ruler from obstructing the line of sight. Rotate the eyepiece so that the scale of the micrometer is parallel to the micrometer of the stage, and a certain stage overlaps the ruled line of the leftmost end of the micrometer and the ruled line of the leftmost end of the micrometer. The position on the ruler. If the right end line of the eye micro ruler does not coincide with any engraved line on the table micro ruler, read the score of the former in the scale of the table micro ruler. Move the micrometer of the stage and make the score line of the left end of the stage micrometer coincide with the score line of the left end of the eye micrometer again to get another reading. In this case, at least 5 readings are required to calculate the average number of times. The average number is multiplied by 10 microns to obtain the actual length represented by each scale of the eye ruler. If S is the average number of readings, E is the number of micrometer scales, and L is the actual length represented by each scale of the micrometer.
L = S / E * 10 microns
Note for this calibration method: the scale line of the micrometer under the high magnification objective lens is very thick, and the scale of the eye micrometer is very thin compared to it, so the line on the left end of the micrometer should be placed on the table micrometer during calibration The left side edge of the left end score line.
Jasmine Essential Oil,Rose Oil For Skin,Rose Massage Oil,Flower Blend Essential Oil
Ji'An ZhongXiang Natural Plants Co.,Ltd. , https://www.zxnaturaloils.com