Intercellular connection
Some cell adhesion molecules are distributed on the plasma membrane of the cell, and some adhesion molecules are used as components of intercellular junctions. The function of different intercellular junction structures is different, such as desmosomes and adhesion junctions bind epithelial cells together; tight The connection can close the cells (such as the secretory cells of alveolar or tubular glands,
Gaps between vascular endothelial cells; gap junctions allow ions. Nutrient molecules and cAMP and other small molecular signaling substances move between cells in contact [Alberts et al., 2002]. Desmosomes in the plasma membrane area where cells contact There are also widely distributed, but they are often co-distributed with the tight junction and adhesion junction structure near the top of the side of the cell. When epithelial cells differentiate in the covered culture, the formation of desmosomes will increase, or when a morphological organization appears, Various types of junction complexes will be formed. This is why it takes too long to spread the culture, and the epithelial cells are not easy to separate. Because many adhesion molecular components in these junction structures are Ca + -dependent, so when or before dissociation Chelating agents such as EDTA are usually added to trypsin.
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