Solid phase extraction device uses eluent elution or heating desorption

Shanghai Haozhuang Instrument Co., Ltd. has developed the LNB brand solid phase extraction device, which is energy-saving and environmentally friendly, leading the country. Shanghai Haozhuang follows the principles of survival by quality, development by technology, efficiency by management, and credibility by service. It has a recognized team of experts in the industry and first-class scientific and technological talents. On the basis of independent research and development of core technologies of scientific instruments, not only have low-temperature and constant-temperature instruments, ultrasonic instruments, cabinet instruments, microbiological instruments, ice-making and refrigeration instruments, laboratory supporting instruments, but also gradually formed various scientific instrument technologies The product pattern of the joint development of reserve products. The solid phase extraction technology of the Hao Zhuang (LNB) brand solid phase extraction device produced by Shanghai Haozhuang Instrument Co., Ltd.-Since the advent of the solid phase extraction method in the late 1970s, SPE columns of various stationary phases have developed very rapidly. In order to continuously adapt the solid phase extraction method to the pretreatment needs of various samples, the solid phase extraction method for different types of compounds has also been gradually improved.

Solid phase extraction (Solid Phase Extraction) is to use a solid adsorbent to adsorb the target compound in the liquid sample, separate it from the sample matrix and interfering compounds, and then elute with the eluent or heat desorption to achieve the separation and enrichment goal The purpose of the compound. As a sample preparation technology, solid phase extraction has been more and more widely used in the laboratory.

SPE is a column chromatography separation process. The separation mechanism, choice of stationary phase and solvent are similar to high performance liquid chromatography (HPLC). However, the particle size of the SPE column packing (> 40! M) is larger than that of the HPLC packing (3 ~ 10! M). Due to the short column bed and large particle size, SPE column efficiency is much lower than HPLC column. A high-efficiency HPLC column can produce more than 10,000 trays, and 10-50 trays can be obtained from an SPE column. Therefore, SPE can only separate compounds with very different properties. In this case, instead of isocratic, a typical SPE separation is performed in a digital switching mode (digitaIon-offmode). This means that with a step gradient, the analyte is either firmly adsorbed by the stationary phase or not retained at all. Another difference from HPLC is that the SPE column is disposable.

The characteristics of the SPE column itself determine its scope of application. SPE technology with lower separation efficiency is mainly used to process samples. The objectives to be achieved with the help of SPE are: (1) remove interfering substances from the sample for later analysis; (2) enrich trace components to improve analytical sensitivity; (3) change the sample solvent to make it compatible with the analysis The methods are matched; (4) In-situ derivation; (5) Desalination of the sample; (6) Facilitate the storage and transportation of the sample.

Among the above points, purification and enrichment are the most important.

Shanghai Haozhuang Instrument Co., Ltd. is a professional laboratory instrument equipment, biochemical instrument equipment, ultrasonic instrument equipment, test box experiment environment instrument equipment manufacturer, research and development, production, sales Haozhuang (LNB) brand instrument-intelligent constant temperature and humidity culture Box, sterile homogenizer, low temperature and constant temperature, welcome to consult and buy! Shanghai Haozhuang Instrument has first-class scientific and technological talents, carefully build high-quality instruments for you, customers are welcome to buy! Reasonable price, quality guarantee, lifetime maintenance, provide customers Fast, professional and high-quality systematic service, your inquiry and purchase are welcome!

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