Technical indicators of the gene amplification instrument PCR instrument

The PCR technique of the PCR instrument of the LNB brand gene amplification instrument of Shanghai Xinzhuang Instrument Co., Ltd. illustrates that semi-reserved replication of DNA is an important pathway for biological evolution and passage. Double-stranded DNA can be degenerated into a single strand under the action of various enzymes. Under the participation of DNA polymerase and promoter, the same two molecules of mussels are replicated according to the principle of base complementary pairing. In the polymerase chain reaction experiment, it was found that DNA can undergo denaturing and melting at high temperatures, and can be renatured into a double strand when the temperature is lowered. Therefore, by controlling the denaturation and renaturation of DNA by temperature changes, and designing primers as promoters, DNA polymerase and dNTP can be added to complete the in vitro replication of specific genes.
However, DNA polymerases are inactivated at high temperatures. Therefore, the addition of new DNA polymerases per cycle is not only cumbersome, but also expensive, which limits the application and development of PCR technology. It is found that the heat-resistant DNA polymerization enzyme-Taq enzyme has a milestone for the application of PCR. The enzyme can withstand high temperatures above 90 °C without inactivation, and does not require enzymes per cycle, making the PCR technology very simple. At the same time, the cost is greatly reduced, and the PCR technology can be applied in a large amount and gradually applied to the clinic.
Shanghai Xinzhuang Instrument Co., Ltd. Xinzhuang (LNB) brand gene amplification instrument PCR instrument PCR works:
Similar to the natural replication process of DNA, its specificity depends on oligonucleotide primers that are complementary to both ends of the target sequence. PCR consists of three basic reaction steps: denaturation-annealing-extension: 1 denaturation of template DNA: template DNA is heated to about 93 °C, and then polymerase chain reaction, the template DNA is double-stranded or PCR amplified. The formed double-stranded DNA dissociates into a single strand so that it binds to the primer to prepare for the next round reaction; 2 anneals (refolds) of the template DNA and the primer: the template DNA is denatured into a single strand by heating, and the temperature Down to 55 °C, the primers are paired with the complementary sequence of the single strand of the template DNA; 3 primer extension: DNA template--primer conjugate under the action of Taq DNA polymerase, using dNTP as the reaction material, the target sequence is template, according to The principle of base pairing and semi-reserved replication, synthesizing a new semi-reserved replication chain that is complementary to the template DNA strand, repeating the cyclic denaturation-annealing-extension process, and obtaining more "semi-reserved replication strands", and this A new chain can be used as a template for the next cycle. It takes 2 to 4 minutes to complete each cycle, and the amplification of the gene to be amplified can be amplified several million times in 2 to 3 hours.
For more information about the PCR technology of the PCR instrument, please contact Shanghai Xinzhuang Senior Engineer, Shanghai Xinzhuang Instrument Co., Ltd. to produce and sell anaerobic incubator series products. The company has a good market reputation, professional sales and Technical service team, with the experience of operating anaerobic incubator series for many years, change the information about anaerobic incubator, please contact Shanghai Xinzhuang Instrument Co., Ltd. for details!
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