Precautions for cleaning the ELISA kit Reagents should be stored according to the label instructions and returned to room temperature before use. The diluted standard should be discarded and cannot be stored. (2) The slats not used in the experiment should be immediately put back into the packaging bag and sealed to prevent deterioration. (3) Other reagents that are not used should be packaged or covered. Do not mix reagents of different batches. Use before warranty. (4) Use a disposable tip to avoid cross-contamination. When aspirating the stop solution and the substrate A and B, avoid using a metallized sample applicator. (5) Dispose of the washing liquid in a clean plastic container. Mix all the ingredients and samples in the kit thoroughly before use. (6) Wash the enzyme plate should be fully patted dry, do not put the absorbent paper directly into the enzyme standard reaction well to absorb water. (7) Substrate A should be volatilized to avoid opening the lid for a long time. Substrate B is sensitive to light and avoids prolonged exposure to light. Avoid contact with hands and be toxic. The OD value should be read immediately after the experiment is completed. (8) The order of adding the reagents should be the same to ensure that all the reaction wells are incubated for the same time. (9) The incubation operation is carried out according to the time indicated in the instruction, the amount of liquid addition, and the order.
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